Lactate Dehydrogenase (LDH)
| Product Name | Lactate Dehydrogenase |
| Source | Porcine Heart |
| Catalogue Number | 350-80 |
| Purity | Purified |
| Form | Liquid |
| Activity | > 5,000 U/mL (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4. |
| Protein | > 15 mg/mL (Coomassie) |
| Specific Activity | > 200 U/mg protein |
| Contaminants | AST/SGOT: < 0.1% ALT/SGPT: < 0.06% MDH: < 0.06% PK: < 0.06% |
| Appearance | Cloudy, white suspension |
| Formulation | Suspension in 2.5 M ammonium sulfate with 0.1 M sodium phosphate, pH 7.0. |
| Recertification | 2 years |
| Storage | 2-8°C |
| Reaction | Catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back. |
| Synonyms | LDH, Lactic Dehydrogenase |
| Molecular Weight | ~136,700 (Huston et al. 1972) |
| CAS Number | 9001-60-9 |
| E.C. Number | 1.1.1.27 |
Lee Biosolutions produces Porcine Heart Lactate Dehydrogenase (LDH) for medical research and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Lactate dehydrogenase (LDH) is an enzyme (EC 1.1.1.27) present in a wide variety of organisms, including plants and animals. Lactate Dehydrogenase catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. LD can also catalyze the oxidation of hydroxybutyrate, Lactate Dehydrogenase (LDH) is occasionally called Hydroxybutyrate Dehydrogenase (HBD).
Lactate Dehydrogenase enzyme is also found in cerebrospinal fluid where high levels of lactate dehydrogenase in cerebrospinal fluid are often associated with bacterial meningitis. High levels of Lactate Dehydrogenase (LDH) enzyme can also be found in cases of viral meningitis, generally indicating the presence of encephalitis and poor prognosis.
Lee Biosolutions, Inc
