Lactate Dehydrogenase (LDH)

Product Name	Lactate Dehydrogenase
Source	Porcine Heart
Catalogue Number	350-80
Purity	Purified
Form	Liquid
Activity	> 5,000 U/mL (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Protein	> 15 mg/mL (Coomassie)
Specific Activity	> 200 U/mg protein
Contaminants	AST/SGOT: < 0.1% ALT/SGPT: < 0.06% MDH: < 0.06% PK: < 0.06%
Appearance	Cloudy, white suspension
Formulation	Suspension in 2.5 M ammonium sulfate with 0.1 M sodium phosphate, pH 7.0.
Recertification	2 years
Storage	2-8°C
Reaction	Catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Synonyms	LDH, Lactic Dehydrogenase
Molecular Weight	~136,700 (Huston et al. 1972)
CAS Number	9001-60-9
E.C. Number	1.1.1.27