Leukocyte Esterase, Unsonicated
| Product Name | Whole Leukocyte Esterase |
| Source | Human Leukocytes |
| Catalogue Number | 341-10 |
| Purity | Blood Fraction |
| Form | Liquid |
| Activity | Esterase: 5.0 - 6.0 U/mL |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole of methoxy succinyl-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5 (In the presence of 100 mM Tris-HCl 500 mM sodium chloride and 0.2% Triton X-100) |
| Dipstick Assay | On lysing the leukocytes, 0.45 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip® 10 SG or Siemens Multistix® 10 SG test strips. |
| Formulation | Suspension in 154 mM sodium chloride. |
| Appearance | Milky, white to off-white suspension |
| Related Products | 910-12 - Bilirubin Conjugate, Ditaurate, Sodium Salt 651-10 - Urobilinogen 343-10 - Esterase from porcine liver |
| Storage | -20°C |
| Recertification | 2 years |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods. |
| Synonyms | Unsonicated White Blood Cell Elastase, WBC Elastase |
Lee Biosolutions is the leading supplier of Human Leukocyte Esterase for use in urine test kits and manufacturing of urine controls by major IVD manufacturers around the world.
On lysing the leukocytes, 0.45 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip 10 with SG or Siemens Multistix 10 SG strips.
Technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. Single lot sizes from 5 to 50 L.
Leukocyte Esterase is used in a urine test for presence of white blood cells abnormalities associated with infection. Urine tests reveal the presence of granulocyte esterases. The esterases cleave a derivatized pyrazole amino acid ester to liberate derivatized hydroxy pyrazole. This pyrazole then reacts with a diazonium salt to produce a purple color.
White blood cells in the urine usually indicate a urinary tract infection. Human leukocytes esterase test detects human esterase, an enzyme released by white blood cells. Human White blood cells are produced in the bone marrow and help to defend the body against infectious disease and foreign materials as part of the immune system. Human Leukocytosis is very common in acutely ill patients. It occurs in response to a wide variety of conditions, including viral, bacterial, fungal, or parasitic infection, cancer, and exposure to certain medications or chemicals.
Human leukocytes are used in routine dipstick tests and urine controls. If this Human leukocyte test is positive, the urine should be examined microscopically for the presence of white blood cells and other abnormalities associated with infection.
Lee Biosolutions, Inc
