Lipase
| Product Name | Lipase |
| Source | Porcine Pancreas |
| Catalogue Number | 400-30 |
| Purity | Purified |
| Form | Lyophilized |
| Formulation | Lyophilized from 20mM Tris, 154mM NaCl, 3mM CaCl2, 1mM Benzamidine Chloride, pH 7.0 |
| Activity | ≥ 30 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole per minute of 1, 2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester in the presence of colipase, bile salt, and calcium chloride at 37°C |
| Protein | ≥ 0.3 mg protein/mg solid (Coomassie) |
| Specific Activity | ≥ 90 U/mg protein |
| Contaminants | a-Amylase: < 1.8% Protease: < 0.03% Cholesterol Esterase: <3% Ammonia: < 0.02 micromole/mg |
| Solubility | Opalescent, light yellow to yellow solution (10 mg/mL saline) |
| Appearance | Off-white to brown powder |
| Reconstitution | > 1 mg/mL in tris buffered saline, 1 mM calcium chloride, 1% BSA, pH 8.0 is recommended. |
| Storage | -20°C |
| Recertification | Three years from date of manufacture |
| Synonyms | Triacylglycerol Acylhydrolase,Triacylglycerol lipase, PPL |
| Molecular Weight | ~48,000 |
| CAS Number | 9001-62-1 |
| E.C. Number | 3.1.1.3 |
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. Bulk quantities from 1-50 million unit single lot sizes. We are bulk manufacturers of diagnostic grade human and animal pancreatic lipase for research and clinical diagnostic manufacturers.
Lipase is a water soluble enzyme that catalyzes the hydrolysis of ester bonds in water insoluble, lipid substrates and is a glycoprotein. Lipase (triacylglycerol acyl hydrolase) fulfills a key function in dietary fat absorption by hydrolysing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids. Pancreatic enzymes have been most studied as part of the treatment for Celiac Disease.
Use: For investigation of pancreatic disorders, pancreatitis, acute and chronic renal disease.
Lee Biosolutions, Inc
