Lipase

Product Name	Lipase
Source	Porcine Pancreas
Catalogue Number	400-30
Purity	Purified
Form	Lyophilized
Formulation	Lyophilized from 20mM Tris, 154mM NaCl, 3mM CaCl2, 1mM Benzamidine Chloride, pH 7.0
Activity	≥ 30 U/mg (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the hydrolysis of one micromole per minute of 1, 2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester in the presence of colipase, bile salt, and calcium chloride at 37°C
Protein	≥ 0.3 mg protein/mg solid (Coomassie)
Specific Activity	≥ 90 U/mg protein
Contaminants	a-Amylase: < 1.8% Protease: < 0.03% Cholesterol Esterase: <3% Ammonia: < 0.02 micromole/mg
Solubility	Opalescent, light yellow to yellow solution (10 mg/mL saline)
Appearance	Off-white to brown powder
Reconstitution	> 1 mg/mL in tris buffered saline, 1 mM calcium chloride, 1% BSA, pH 8.0 is recommended.
Storage	-20°C
Recertification	Three years from date of manufacture
Synonyms	Triacylglycerol Acylhydrolase,Triacylglycerol lipase, PPL
Molecular Weight	~48,000
CAS Number	9001-62-1
E.C. Number	3.1.1.3