Creatine Kinase MB (CK-MB)

Catalogue Number: 190-25
Product Name Creatine Kinase MB Isoenzyme
Source Human Heart
Catalogue Number 190-25
Purity Purified (Control Grade)
Form Lyophilized
Activity > 25 U/mg solid (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C.
Protein > 0.25 mg protein/mg (Coomassie)
Specific Activity Report in U/mg protein
Isozyme Content CK-MB: > 99% (Helena QuickGel® CK)
CK-MM: < 1%
CK-BB: < 1%
Contaminants GOT/AST: < 1% (Dimension® Clinical Chemistry System)
LDH: < 1%
Ammonia: < 0.1 micromole/mg solid
Formulation Lyophilized from 10 mM tris-succinate with 2 mM EDTA and 2 mM DTT, pH 7.5.
Reconstitution Tris buffered saline with 1% BSA, 1 mM DTT and 1 mM EDTA.
Appearance Light yellow to tan powder.
Recertification 3 years
Storage -20°C
Infectious Disease Testing Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms CKMB, CK-MB
Molecular Weight 84,000
CAS Number 9001-15-4
E.C. Number

Lee Biosolutions is the leading supplier of Human Creatine Kinase MB (CK-MB) isoenzyme for diagnostic manufacturing and research purposes.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Human Creatine Kinase is one of the most important myocardial markers (in spite of not being altogether cardiac-specific), with well-established roles in confirming acute myocardial infarction (AMI). It can also used for monitoring reperfusion during thrombolytic therapy following AMI. Testing is performed when the total Human Creatine Phosphokinase (CPK) level is elevated. Isoenzyme testing can help differentiate the source of the damaged tissue. Human CPK enzyme consists of two subunits, which can be either B (brain type) or M (muscle type). Three different isoenzymes: Creatine Kinase MM, BB, and MB isoenzymes exist.

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