Creatine Kinase MB (CK-MB)

Product Name	Creatine Kinase MB Isoenzyme
Source	Human Heart
Catalogue Number	190-25
Purity	Purified (Control Grade)
Form	Lyophilized
Activity	> 25 U/mg solid (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C.
Protein	> 0.25 mg protein/mg (Coomassie)
Specific Activity	Report in U/mg protein
Isozyme Content	CK-MB: > 99% (Helena QuickGel® CK) CK-MM: < 1% CK-BB: < 1%
Contaminants	GOT/AST: < 1% (Dimension® Clinical Chemistry System) LDH: < 1% Ammonia: < 0.1 micromole/mg solid
Formulation	Lyophilized from 10 mM tris-succinate with 2 mM EDTA and 2 mM DTT, pH 7.5.
Reconstitution	Tris buffered saline with 1% BSA, 1 mM DTT and 1 mM EDTA.
Appearance	Light yellow to tan powder.
Recertification	3 years
Storage	-20°C
Infectious Disease Testing	Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms	CKMB, CK-MB
Molecular Weight	84,000
CAS Number	9001-15-4
E.C. Number	2.7.3.2