Avidin
| Product Name | Avidin |
| Source | Chicken Egg White |
| Catalogue Number | 102-90 |
| Purity | Highly Purified |
| Form | Lyophilized |
| Protein | > 0.90 mg protein/mg solid |
| Activity | > 10 U/mg solid |
| Unit Definition | One unit will bind one microgram of d-biotin. |
| Moisture Content | Reported |
| Related Products | 480-20 - Alkaline Phosphatase from calf intestines 277-03 - Glucose Oxidase 439-25 - Ovalbumin from chicken egg whites |
| Application | Egg white Avidin can be used in assays for functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol. |
| Recertification | 3 years |
| Storage | -20°C |
| Molecular Weight | 64,000 (tetramer of 16,000 MW subunits) |
| CAS Number | 1405-69-2 |
| EC Number | 215-783-6 |
| Gene | AVD |
| Gene ID | 396260 |
| Accession No | P02701 |
Lee Biosolutions produces proteins from chicken egg whites, including Albumin, Lysozyme and Avidin, for research, life science and manufacturing applications.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Avidin is a tetrameric protein. In chicken egg white, avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer) each of which can bind to biotin (Vitamin B7, vitamin H) with a high degree of affinity and specificity.
Avidin's affinity for biotin is exploited in wide ranging biochemical assays, including Western Blot, ELISA, ELISPOT and pull-down assays. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using an avidin-based support.
Avidin and biotin are routinely conjugated to indicator enzymes, such as alkaline phosphatase and horseradish peroxidase for use in diagnostic kit development and manufacturing.
Lee Biosolutions, Inc
