Alanine Aminotransferase (ALT/GPT)
| Product Name | Alanine Aminotransferase |
| Source | Porcine Heart |
| Catalogue Number | 310-20 |
| Purity | Purified |
| Form | Liquid |
| Activity | 1,600 - 2,400 U/mL (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4. |
| Protein | Reported in mg protein/mL (Coomassie) |
| Specific Activity | Reported in U/mg protein |
| Contaminants | CPK: < 0.1% GLDH: < 0.01% AST/GOT: < 0.5% LDH: < 0.1% Protease: < 0.02 U/mg (< 0.001 O.D./8 min.) |
| Appearance | Yellow to green suspension |
| Formulation | Suspension in 1.2 M ammonium sulfate. |
| Related Products | 310-30 - Alanine Aminotransferase (Lyophilized) from porcine heart 490-20 - Alkaline Phosphatase from porcine kidney 300-20 - Aspartate Aminotransferase from porcine heart |
| Storage | 2-8°C |
| Recertification | 2 years |
| Molecular Weight | ~100,000 |
| Product Specificity | Liver damage or disease |
| Synonyms | Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT |
| CAS Number | 9000-86-6 |
| E.C. Number | 2.6.1.2 |
| Gene | GPT, AAT1, GPT1 |
Lee Biosolutions is the leading producer of Glutamate Pyruvate Transaminase/Alanine Transaminase (GPT/ALT), used in multi-analyte clinical chemistry controls and calibrators for major IVD manufacturers around the world.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
The Alanine Aminotransferase (ALT) blood test is typically used to detect liver injury. It is often ordered in conjunction with Aspartate Aminotransferase (AST) or as part of a liver panel to screen for and/or help diagnose liver disease. AST and ALT are considered to be two of the most important tests to detect liver injury, although ALT is more specific than AST. Sometimes AST is compared directly to ALT and an AST/ALT ratio is calculated. This ratio may be used to distinguish between different causes of liver damage.
Elevated levels of Glutamate Pyruvate Transaminase/Alanine Transaminase (GPT/ALT) often suggest the existence of other medical problems such as alcoholic or viral hepatitis, congestive heart failure, liver damage, biliary duct problems, infectious mononucleosis, or myopathy.
Human GPT/ALT is an enzyme that catalyzes the reversible transfer of an amino group from alanine to a-ketoglutarate. Human Alanine Aminotransferase is found in serum and in various bodily tissues but is most commonly associated with the liver. Also called Serum Glutamate Pyruvate Transaminase (SGPT) or Alanine Aminotransferase (ALAT).
Lee Biosolutions, Inc
