Alanine Aminotransferase (ALT/GPT)

Product Name Alanine Aminotransferase
Source Porcine Heart
Catalogue Number 310-20
Purity Purified
Form Liquid
Activity 1,600 - 2,400 U/mL (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4.
Protein Reported in mg protein/mL (Coomassie)
Specific Activity Reported in U/mg protein
Contaminants CPK: < 0.1%
GLDH: < 0.01%
AST/GOT: < 0.5%
LDH: < 0.1%
Protease: < 0.02 U/mg (< 0.001 O.D./8 min.)
Appearance Yellow to green suspension
Formulation Suspension in 1.2 M ammonium sulfate.
Related Products 310-30 - Alanine Aminotransferase (Lyophilized) from porcine heart
490-20 - Alkaline Phosphatase from porcine kidney
300-20 - Aspartate Aminotransferase from porcine heart
Storage 2-8°C
Recertification 2 years
Molecular Weight ~100,000
Product Specificity Liver damage or disease
Synonyms Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT
CAS Number 9000-86-6
E.C. Number
Gene GPT, AAT1, GPT1