Very Low Density Lipoprotein (VLDL)
| Product Name | Very Low Density Lipoprotein |
| Source | Human Plasma |
| Catalogue Number | 365-10 |
| Purity | Plasma Fraction |
| Form | Liquid |
| Triglycerides | > 8,000 mg/dL (Enzymatic) |
| Total Cholesterol | Reported in mg/dL (Enzymatic) |
| Triglyceride Ratio | > 2.0 (Triglycerides/Total Cholesterol) |
| Appearance | Yellow to orange liquid |
| Handling | - Thaw quickly, in a 37°C water bath, removing as soon as thawed. - Store at 2 – 8°C for up to one week, -20°C for up to three months, and up to 24 months at -70°C. - Dilute with 0.15 M sodium chloride containing 0.01 mM EDTA. |
| Related Products | 361-10 - HDL Concentrate from human plasma 360-10 - LDL Concentrate from human plasma 361-56 - Triglyceride Concentrate from human plasma |
| Storage | -20°C (up to three months) -70°C (more than three months) |
| Recertification | 2 years |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods. |
| Synonyms | VLDL, VLDL-C, Concentrate, Fraction |
| UNSPSC code | 51131909 |
| Country of Origin | United States |
| References | Acta Biomaterialia Volume 71, 15 April 2018, Pages 420-431 |
Lee Biosolutions is world leader in the manufacturing of human plasma Very Low Density Lipoprotein (VLDL) and other cholesterol/triglyceride products for research and in vitro diagnostic (IVD) manufacturing uses.
Custom LDL/HDL and triglyceride ratios, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Procedure: Ultracentrifugation performed to isolate Human Very Low Density lipoprotein (VLDL). Very Low Density Lipoprotein levels have been correlated with accelerated rates of atherosclerosis, and are elevated in a number of diseases and metabolic states. Human VLDL Inhibits DNA synthesis in lymphocytes activated by the nonspecific mitogen concanavalin A (Con A). Human VLDL are the next step down from chylomicrons in terms of size and lipid content. It transports endogenous triglycerides, phospholipids, cholesterol and cholesteryl esters. This functions as the body's internal transport mechanism for lipids.
VLDL is defined as the lipoprotein fraction with the density range of 0.93 to 1.006 g/mL. It is spherical particle and in normal individuals ranges 30 to 80 nm in diameter. The molecular weight is 10 to 8 107. Compare with LDL particles, VLDL particles are heterogeneous in size and composition. VLDL particles can be separated in Sf 20-60 and Sf60-400. VLDL consists of protein 10%, phospholipid 19%, free cholesterol 7%, cholesterol ester 10% and triglycerides 56%. Apolipoprotein contribution% apoB 37 apoC 50 apoE 13 Apo B-48 is the sole constituent apolipoprotein on VLDL. It is presumed that the cholesteryl esters and triglycerides form the hydrophobic core, which is surrounded by a surface coat of apolipoprotein B, free cholesterol and polar phospholipid components oriented toward the aqueous medium.
Lee Biosolutions, Inc
