Pyruvate Kinase (PK)
Catalogue Number: 500-20
Description
| Product Name | Pyruvate Kinase |
| Source | Rabbit Muscle |
| Catalogue Number | 500-20 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 200 U/mg |
| Unit Definition | One unit will catalyze the conversion of 1.0 micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.6 at 37°C |
| Protein | > 0.4 mg protein/mg solid (A280nm, E0.1%=0.54) |
| Specific Activity | Report U/mg protein |
| Contaminants | CPK: < 1.0% LDH: < 0.01% |
| Appearance | White to off-white powder |
| Formulation | Powder, lyophilized from tris chloride, pH 7.7. |
| Related Products | 490-76 - PGM from rabbit muscle 191-50 - CPK from rabbit muscle 276-50 - G3PDH from rabbit muscle |
| Storage | -20°C |
| Recertification | 3 years |
| Synonyms | ATP:Pyruvate 2-0-phosphotransferase, PK |
| Action | Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This transfer yields one molecule of pyruvate and one molecule of ATP. |
| Application | Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes. |
| Molecular Weight | 237 kDa (as a tetramer of four equal subunits 57 kDa) |
| Reaction | ATP + pyruvate = ADP + phosphoenolpyruvate |
| E.C. Number | 2.7.1.40 |
| CAS Number | 9001-59-6 |
| Gene | PKM, PKM2 |
| Gene ID | 100008676 |
| Accession No | P14618 |
| References | Vascular Pharmacology, Volume 59, Issues 3–4, September–October 2013, Pages 76-82 |
Applications
Overview
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Pyruvate Kinase is an enzyme involved in glycolysis. Pyruvate Kinase (PK) catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding a pyruvate molecule and producing one molecule ATP.
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