Pyruvate Kinase (PK)

Product Name	Pyruvate Kinase
Source	Rabbit Muscle
Catalogue Number	500-20
Purity	Purified
Form	Lyophilized
Activity	> 200 U/mg
Unit Definition	One unit will catalyze the conversion of 1.0 micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.6 at 37°C
Protein	> 0.4 mg protein/mg solid (A_280nm, E^0.1%=0.54)
Specific Activity	Report U/mg protein
Contaminants	CPK: < 1.0% LDH: < 0.01%
Appearance	White to off-white powder
Formulation	Powder, lyophilized from tris chloride, pH 7.7.
Related Products	490-76 - PGM from rabbit muscle 191-50 - CPK from rabbit muscle 276-50 - G3PDH from rabbit muscle
Storage	-20°C
Recertification	3 years
Synonyms	ATP:Pyruvate 2-0-phosphotransferase, PK
Action	Pyruvate kinase catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP. This transfer yields one molecule of pyruvate and one molecule of ATP.
Application	Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes.
Molecular Weight	237 kDa (as a tetramer of four equal subunits 57 kDa)
Reaction	ATP + pyruvate = ADP + phosphoenolpyruvate
E.C. Number	2.7.1.40
CAS Number	9001-59-6
Gene	PKM, PKM2
Gene ID	100008676
Accession No	P14618
References	Vascular Pharmacology, Volume 59, Issues 3–4, September–October 2013, Pages 76-82