Alkaline Phosphatase (CIP/CIAP)
Catalogue Number: 480-20
Description
| Product Name | Alkaline Phosphatase |
| Source | Calf Intestines |
| Catalogue Number | 480-20 |
| Purity | Highly Purified |
| Form | Lyophilized |
| Activity | > 210 U/mg (Siemens Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will convert one micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate per minute at 37°C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35 and 37°C. |
| Protein | > 0.4 mg protein/mg (Coomassie) |
| Specific Activity | > 280 U/mg protein (Typically > 1,000 U/mg protein) |
| Contaminants | Ammonia: < 1.0 micromole/mg |
| Formulation | Lyophilized from tris-chloride and magnesium chloride, pH 8.3 |
| Appearance | Off-white to tan powder |
| Solubility | Clear, colorless in 1 mg/mL saline. |
| Reconstitution | Tris buffered saline with 1 mM magnesium chloride, 0.2 mM zinc chloride, and 1% BSA, pH 8.0. |
| Related Products | 490-20 - Alkaline Phosphatase from porcine kidney 310-30 - Alanine Aminotransferase from porcine heart 300-20 - Aspartate Aminotransferase from porcine heart |
| Storage | -20°C |
| Recertification | 3 years |
| Molecular Weight | Consists of two identical subunits of 65kDa |
| Synonyms | CIP, CIAP, ALP, Orthophosphoric-Monoester Phosphohydrolase |
| CAS Number | 9001-78-9 |
| E.C. Number | 3.1.3.1 |
| Gene | ALPI, BOS_2150, IAP |
| Gene ID | 280993 |
| References | J Pediatr Gastroenterol Nutr. 2011 Oct; 53(4): 371–379. |
Applications
Overview
Lee Biosolutions is the leading producer of Calf Intestine Alkaline Phosphatase (CIAP, ALP, ALKP) for research and bulk manufacturing for clinical diagnostics.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta.
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