Alkaline Phosphatase (CIP/CIAP)

Product Name Alkaline Phosphatase
Source Calf Intestines
Catalogue Number 480-20
Purity Highly Purified
Form Lyophilized
Activity > 210 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition One unit will convert one micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate per minute at 37°C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35 and 37°C.
Protein > 0.4 mg protein/mg (Coomassie)
Specific Activity > 280 U/mg protein (Typically > 1,000 U/mg protein)
Contaminants Ammonia: < 1.0 micromole/mg
Formulation Lyophilized from tris-chloride and magnesium chloride, pH 8.3
Appearance Off-white to tan powder
Solubility Clear, colorless in 1 mg/mL saline.
Reconstitution Tris buffered saline with 1 mM magnesium chloride, 0.2 mM zinc chloride, and 1% BSA, pH 8.0.
Related Products 490-20 - Alkaline Phosphatase from porcine kidney
310-30 - Alanine Aminotransferase from porcine heart
300-20 - Aspartate Aminotransferase from porcine heart
Storage -20°C
Recertification 3 years
Molecular Weight Consists of two identical subunits of 65kDa
Synonyms CIP, CIAP, ALP, Orthophosphoric-Monoester Phosphohydrolase
CAS Number 9001-78-9
E.C. Number
Gene ALPI, BOS_2150, IAP
Gene ID 280993
References J Pediatr Gastroenterol Nutr. 2011 Oct; 53(4): 371–379.