Alkaline Phosphatase (CIP/CIAP)

Product Name	Alkaline Phosphatase
Source	Calf Intestines
Catalogue Number	480-20
Purity	Highly Purified
Form	Lyophilized
Activity	> 210 U/mg (Siemens Dimension® Clinical Chemistry System)
Unit Definition	One unit will convert one micromole of p-nitrophenylphosphate to p-nitrophenol and phosphate per minute at 37°C in the presence of AMP (2-amino-2-methyl-1-propanol) at pH 10.35 and 37°C.
Protein	> 0.4 mg protein/mg (Coomassie)
Specific Activity	> 280 U/mg protein (Typically > 1,000 U/mg protein)
Contaminants	Ammonia: < 1.0 micromole/mg
Formulation	Lyophilized from tris-chloride and magnesium chloride, pH 8.3
Appearance	Off-white to tan powder
Solubility	Clear, colorless in 1 mg/mL saline.
Reconstitution	Tris buffered saline with 1 mM magnesium chloride, 0.2 mM zinc chloride, and 1% BSA, pH 8.0.
Related Products	490-20 - Alkaline Phosphatase from porcine kidney 310-30 - Alanine Aminotransferase from porcine heart 300-20 - Aspartate Aminotransferase from porcine heart
Storage	-20°C
Recertification	3 years
Molecular Weight	Consists of two identical subunits of 65kDa
Synonyms	CIP, CIAP, ALP, Orthophosphoric-Monoester Phosphohydrolase
CAS Number	9001-78-9
E.C. Number	3.1.3.1
Gene	ALPI, BOS_2150, IAP
Gene ID	280993
References	J Pediatr Gastroenterol Nutr. 2011 Oct; 53(4): 371–379.