Isocitrate Dehydrogenase (IDH, NADP+)
Catalogue Number: 341-01
Description
| Availability | Discontinued- alternative 341-02 |
| Product Name | Isocitrate Dehydrogenase |
| Source | Porcine Heart |
| Catalogue Number | 341-01 |
| Form | Lyophilized |
| Purity | Partially Purified |
| Activity | > 0.3 U/mg solid |
| Unit Definition | One unit will catalyze the oxidation of one micromole of isocitrate and the reduction of one micromole of NADP per minute at pH 7.4 and 37°C. |
| Protein | Reported mg protein/mg solid (Coomassie) |
| Specific Activity | Report U/mg protein |
| Appearance | Tan to brown powder |
| Solubility | Report (1 mg/mL saline) |
| Reconstitution | - > 1 mg /mL 100 mM triethanolamine chloride, 50 mM sodium chloride, 1% BSA, pH 8.0 - Aliquot and freeze at -20°C after reconstitution |
| Recertification | 3 years |
| Storage | -20°C |
| Synonyms | NADP, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase |
| CAS Number | 9028-48-2 |
| E.C. Number | 1.1.1.42 |
| Gene | IDH1, ICDH |
| Reaction | Isocitrate + NADP(+) <=> 2-oxoglutarate + CO(2) + NADPH |
Applications
Overview
Lee Biosolutions producer and supplier of Isocitrate Dehydrogenase-ICDH - NADP is from porcine heart for laboratory research and manufacturing clinical diagnostic assays.
ICDH is available in bulk and research quantities. Inquire on custom buffers and specifications.
Isocitrate dehydrogenase is an enzyme that participates in the citric acid cycle. ICDH catalyzes the third step of the cycle: the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate (a-ketoglutarate) and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate (a secondary alcohol) to oxalosuccinate (a ketone), followed by the decarboxylation of the carboxyl group beta to the ketone, forming alpha-ketoglutarate.
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