Alkaline Phosphatase (ALP)

Catalogue Number: 490-21
Availability Discontinued - See 490-20 for a suggested alternative
Product Name Alkaline Phosphatase
Source Porcine Kidney
Catalogue Number 490-21
Purity Highly Purified
Form Lyophilized
Activity > 750 U/mg solid (Dimension® Clinical Chemistry System)
Unit Definition One unit catalyzes the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37°C and pH 10.35 in the presence of 2-amino-2-methyl-1-propanol.
Protein > 0.5 mg protein/mg (Coomassie)
Specific Activity > 1,600 U/mg protein
Contaminants gGT: < 0.01%
AST/SGOT: < 0.01%
LDH: < 0.01%
Ammonia: < 0.01 micromole/mg
Formulation Lyophilized from tris-chloride, with magnesium chloride and zinc chloride, pH 8.0.
Appearance White to tan powder
Related Products 490-20 - Alkaline Phosphatase from porcine kidney
310-30 - Alanine Aminotransferase from porcine heart
300-20 - Aspartate Aminotransferase from porcine heart
Storage -20°C
Recertification 2 years
Synonyms ALP, Orthophosphoric-Monoester Phosphohydrolase
CAS Number 9001-78-9
E.C. Number

Lee Biosolutions is the leading producer of high purity porcine kidney Alkaline Phosphatase (ALP) for research, life science and diagnostic manufacturing.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Alkaline Phosphatase is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is 10.0 in standard conditions.

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