Alanine Aminotransferase (ALT/GPT)
| Product Name | Alanine Aminotransferase |
| Source | Porcine Heart |
| Catalogue Number | 310-30 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 90 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the transamination of one micromole per minute of L-alanine to a-ketoglutarate, forming L-glutamate and pyruvate, at 37°C and pH 7.4. |
| Protein | > 0.8 mg protein/mg solid (A280) |
| Specific Activity | > 80 U/mg protein |
| Contaminants | CPK: < 0.1% GLDH: < 0.01% AST/GOT: < 0.5% LDH: < 0.1% Protease: < 0.02 U/mg (< 0.001 O.D./8 min.) Ammonia: < 0.5 micromole/mg |
| Formulation | Lyophilized from tris-chloride, pH 7.0 with sodium chloride, EDTA, DTT, a-ketoglutarate and pyridoxal-5-phosphate. |
| Appearance | Yellow to yellow-green powder |
| Solubility | Clear, colorless to yellow-green solution (1 mg/mL saline) |
| Reconstitution | Clear, yellow-green (10 mg/mL saline) |
| Related Products | 480-20 - Alkaline Phosphatase from calf intestine 300-20 - Aspartate Aminotransferase from porcine heart 350-50 - Lactate Dehydrogenase from chicken heart |
| Storage | -20°C |
| Recertification | 3 years |
| Molecular Weight | ~100,000 |
| Product Specificity | Liver damage or disease |
| Synonyms | Glutamate Pyruvate Transaminase, Alanine Transaminase, serum glutamic-pyruvic transaminase, SGPT, GPT, ALT, ALAT |
| CAS Number | 9000-86-6 |
| E.C. Number | 2.6.1.2 |
| Gene | GPT, AAT1, GPT1 |
| Accession No | P13191 |
Lee Biosolutions is the leading producer of Porcine Heart Glutamic-Pyruvate Transaminase (GPT) enzyme, also known as Alanine transaminase (ALT) and alanine aminotransferase for research and clinical diagnostic assays for liver disease.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Alanine transaminase (GPT/ALT) is used in multi-analyte clinical chemistry controls and calibrators for major IVD manufacturers around the world.
The alanine aminotransferase (ALT) blood test is typically used to detect liver injury. GPT/ALT test is often ordered in conjunction with aspartate aminotransferase (AST) or as part of a liver panel to screen for and/or help diagnose liver disease. AST and ALT are considered to be two of the most important tests to detect liver injury, although ALT is more specific than AST. Sometimes AST is compared directly to ALT and an AST/ALT ratio is calculated. This ratio may be used to distinguish between different causes of liver damage.
GPT values are often compared to the results of other tests such as alkaline phosphatase (ALP), total protein, and bilirubin to help determine which form of liver disease is present.
ALT is often used to monitor the treatment of persons who have liver disease, to see if the treatment is working, and may be ordered either by itself or along with other tests for this purpose.
Lee Biosolutions, Inc
