Creatine Kinase MB (CK-MB), Type II
Catalogue Number: 190-24-R2
Description
Product Name | Human Creatine Kinase MB |
Source | Recombinant |
Catalogue Number | 190-24-R2 |
Purity | Highly Purified |
Purity Note | Doublet at 47 kDa |
Form | Liquid |
Expression System | Pichia pastoris (Yeast) |
Protein | > 10 mg/mL (Coomassie) |
Activity | > 10,000 U/mL |
Unit Definition | One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. |
Specific Activity | > 700 U/mg Protein |
Formulation | Solution in 10 mM Tris-HCl buffer containing 50% (v/v) glycerol, 0.5 mM DTT, 0.5 mM EDTA, pH 6.6 – 7.0. |
Product Details | Heterodimer comprised of recombinant, full-length B subunit (accession P12277, 2 – 381) and recombinant M subunit (accession P06732, 2 – 381) |
Storage | -70°C |
Recertification | 5 years |
Synonyms | r-hCKMB II, r-hCK-MB, r-hCK-2 |
Molecular Weight | 84,000 |
E.C. Number | 2.7.3.2 |
CAS Number | 9001-15-4 |
Applications
Overview
Recombinant Human Creatine Kinase MB isoenzyme, CK-MB Isoenzyme BULK QUANTITIES from 50-500 mg single lot sizes.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Recombinant full length Creatine Kinase MB Isoenzyme with amino acid sequence identical to native enzyme. CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 47 kDa is not resolved on most gels. Purified in the enzymatically active form. Reacts with monoclonal antibodies specific to MB isoenzyme in ELISA.
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