Creatine Kinase MB (CK-MB), Type I
Catalogue Number: 190-24-R1
Description
Product Name | Human Creatine Kinase MB |
Source | Recombinant |
Catalogue Number | 190-24-R1 |
Purity | > 95% (SDS-PAGE) |
Form | Liquid |
Expression System | Pichia pastoris (Yeast) |
Protein | > 1.0 mg/mL (Coomassie) |
Activity | > 400 U/mL @ 37°C |
Unit Definition | One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. |
Formulation | Solution in 20 mM potassium phosphate, 1 mM DTT, 50% glycerol (v/v), pH 5 - 6. |
Appearance | Clear, colorless solution |
Additional Info | Heterodimer comprised of recombinant, full-length B subunit (accession P12277, 2 – 381) and recombinant M subunit without the C-terminal lysine (accession P06732, 2 – 380) |
Storage | -70°C |
Recertification | 2 years |
Application | ELISA, Western Blot |
Applications
Overview
Lee Biosolutions sells Recombinant Human Creatine Kinase MB (CK-MB) for ELISA and Western blot applications, among others.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Recombinant full length Creatine Kinase MB isoenzyme without the C-terminal lysine on the M subunit. CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 44 kDa is not resolved on most gels. Purified in the enzymatically active form and reacts with monoclonal antibodies specific to MB isoenzyme in ELISA.
Add to Cart