Alkaline Phosphatase (ALP)
Catalogue Number: 490-20
Description
| Product Name | Alkaline Phosphatase |
| Source | Porcine Kidney |
| Catalogue Number | 490-20 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 50 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit catalyzes the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37°C and pH 10.35 in the presence of 2-amino-2-methyl-1-propanol. |
| Protein | > 0.5 mg protein/mg solid (Coomassie) |
| Specific Activity | > 50 U/mg protein |
| Contaminants | gGT: < 2% AST/SGOT: < 1.0% LDH: < 0.1% Ammonia: < 0.1 micromole/mg |
| Formulation | Lyophilized from tris chloride with magnesium chloride and zinc chloride, pH 8.0. |
| Appearance | Tan powder |
| Solubility | Clear, colorless to tan solution in 1 mg/mL saline. |
| Related Products | 480-20 - Alkaline Phosphatase from calf intestine 310-30 - Alanine Aminotransferase from porcine heart 350-50 - Lactate Dehydrogenase from chicken heart |
| Storage | -20°C |
| Recertification | 3 years |
| Synonyms | ALP, Orthophosphoric-Monoester Phosphohydrolase |
| CAS Number | 9001-78-9 |
| E.C. Number | 3.1.3.1 |
Applications
Overview
Lee Biosolutions is the leading producer of porcine kidney Alkaline Phosphatase (ALP) for research, life science and diagnostic manufacturing.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Alkaline Phosphatase is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is 10.0 in standard conditions.
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