Alkaline Phosphatase (ALP/PLAP)
Catalogue Number: 485-10
Description
| Product Name | Alkaline Phosphatase |
| Source | Human Placenta |
| Catalogue Number | 485-10 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 10 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37°C and pH 10.4 in glycine buffer. |
| Protein | > 0.1 mg protein/mg (Coomassie) |
| Specific Activity | Typically > 25 U/mg protein |
| Formulation | Powder, lyophilized from tris chloride, with magnesium chloride and zinc chloride, pH 7.4. |
| Reconstitution | Tris buffered saline containing 10 mg/mL BSA, 1 mM magnesium chloride, and 0.2 mM zinc chloride, pH 8.0. 1 – 10 mg/mL and dilute as needed. |
| Related Products | 480-20 - Alkaline Phosphatase from calf intestines 490-20 - Alkaline Phosphatase from porcine kidney 270-60 - Ferritin from human placenta |
| Storage | -20°C |
| Recertification | 3 years |
| Specificity | Tumor Marker, seminoma, ovarian cancer |
| Synonyms | ALP, PLAP, Alk Phos |
| CAS Number | 9001-78-9 |
| E.C. Number | 3.1.3.1 |
Applications
Overview
Lee Biosolutions is the leading supplier of Human Placental Alkaline Phosphatase (ALP) for life science, laboratory, research and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids. In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH=10 in standard conditions.
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