Lactate Dehydrogenase 5 (LDH-5)

Product Name Lactate Dehydrogenase 5
Source Human Liver
Catalogue Number 355-50
Form Liquid
Purity Purified
Activity > 1,000 U/mL (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Protein > 1.0 mg protein/mL (Coomassie)
Specific Activity > 200 U/mg protein
Isoenzyme Content LDH-5: > 95% (Helena QuickGel® LD Isoenzyme Electrophoresis)
LDH-1: < 2.0%
LDH-2: < 2.0%
LDH-3: < 2.0%
LDH-4: < 2.0%
Contaminants CPK: < 1%
Formulation Suspension in 3.1 M ammonium sulfate, tris chloride, DTT, and EDTA, pH 8.3.
Storage 2-8°C
Recertification 2 years
Reaction Catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Synonyms LD-5 Isoenzyme, LDH-5 Isoenzyme, 4M Isoenzyme
CAS Number 9001-60-9
E.C. Number