Lactate Dehydrogenase 5 (LDH-5)

Product Name	Lactate Dehydrogenase 5
Source	Human Liver
Catalogue Number	355-50
Form	Liquid
Purity	Purified
Activity	> 1,000 U/mL (Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Protein	> 1.0 mg protein/mL (Coomassie)
Specific Activity	> 200 U/mg protein
Isoenzyme Content	LDH-5: > 95% (Helena QuickGel® LD Isoenzyme Electrophoresis) LDH-1: < 2.0% LDH-2: < 2.0% LDH-3: < 2.0% LDH-4: < 2.0%
Contaminants	CPK: < 1%
Formulation	Suspension in 3.1 M ammonium sulfate, tris chloride, DTT, and EDTA, pH 8.3.
Storage	2-8°C
Recertification	2 years
Reaction	Catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Synonyms	LD-5 Isoenzyme, LDH-5 Isoenzyme, 4M Isoenzyme
CAS Number	9001-60-9
E.C. Number	1.1.1.27