Lactate Dehydrogenase (LDH)

Product Name	Lactate Dehydrogenase
Source	Bovine Heart
Catalogue Number	350-20
Purity	Purified
Form	Lyophilized
Activity	> 100 U/mg or reported in U/vial (Siemens Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Protein	> 0.5 mg protein/mg or reported in mg protein/vial (Coomassie)
Specific Activity	> 100 U/mg protein
Contaminants	AST/GOT: < 0.6% ALT/GPT: < 0.07% Pyruvate Kinase: Typically < 0.1% Ammonia: < 0.1 micromole/mg or reported in micromole/vial
Appearance	White to off-white powder
Solubility	Clear, colorless to tan solution with possible fine particulates
Reconstitution	> 1 mg/mL in phosphate buffered saline
Recertification	3 years
Storage	-20°C
Molecular Weight	~136,700 (Huston et al. 1972)
Synonyms	LAD, LD, L-LDH, (S)-Lactate, LDH
CAS Number	9001-60-9
E.C. Number	1.1.1.27