Lactate Dehydrogenase (LDH)

Product Name	Lactate Dehydrogenase
Source	Bovine Heart
Catalogue Number	350-11
Purity	Purified
Form	Liquid
Activity	2,000 - 2,400 U/mL (Siemens Dimension® Clinical Chemistry System)
Unit Definition	One unit will catalyze the oxidation of one micromole of L-lactate to pyruvate with simultaneous reduction of NAD+ to NADH per minute at 37°C and pH 9.4.
Protein	5 - 20 mg protein/mL (Coomassie)
Specific Activity	> 100 U/mg protein
Contaminants	AST/GOT: < 0.6% ALT/GPT: < 0.07%
Formulation	Suspension in 3.1 M ammonium sulfate
Appearance	White to off-white slurry
Recertification	2 years
Storage	2-8°C
Reaction	Catalyzes the conversion of pyruvate to lactate and back, as it converts NADH to NAD+ and back.
Synonyms	LAD, LD, L-LDH, (S)-Lactate: NAD+ oxidoreductase
Molecular Weight	Appr. 136,700 (Huston et al. 1972)
CAS Number	9001-60-9
E.C. Number	1.1.1.27