Leukocytes, Sonicated

Product Name Sonicated Leukocytes
Source Human Leukocytes
Catalogue Number 342-10
Form Liquid
Purity Blood Fraction
Activity 5.0 - 6.0 U/mL (Elastase)
Unit Definition One unit will catalyze the hydrolysis of one micromole of methoxy succinyl-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5 (In the presence of 500 mM sodium chloride and 0.2% Triton X-100)
Protein > 1.0 mg/mL (Coomassie)
Dipstick Assay Leukocytes: > 2+
Protein: Negative
Glucose: Negative
Blood: Less than trace
Dipstick Protocol 0.9 mL per liter of saline will give a positive reaction for leukocytes with Roche Chemstrip® 10 SG and/or Siemens Multistix® 10 SG test strips.
Formulation Suspension in 154 mM sodium chloride.
Appearance Milky white suspension. May form aggregates due to freeze/thaws.
Handling Sonicate prior to use to disperse aggregates.
Related Products 910-12 - Bilirubin Conjugate, Ditaurate, Sodium Salt
341-10 - Whole Leukocytes from human
343-10 - Esterase from porcine liver
Storage -20°C
Recertification 2 years
Infectious Disease Testing Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods.
Synonyms Immune cells, LE, Elastase