Isocitrate Dehydrogenase (IDH, NADP+)

Product Name Isocitrate Dehydrogenase
Source Porcine Heart
Catalogue Number 341-02
Form Liquid
Purity Partially Purified
Activity > 10 U/mL (Kinetic, spectrophotometric)
Unit Definition One unit will catalyze the oxidation of one micromole of isocitrate and the reduction of one micromole of NADP per minute at pH 7.4 and 37°C.
Protein > 10 mg/mL (Coomassie)
Specific Activity > 1 U/mg protein
Formulation Suspension in 3.3 M ammonium sulfate, 50 mM MES and 1 mM manganese sulfate, pH 6.4.
Appearance Brown suspension
Handling - Mix thoroughly before sampling
- Dilute with 100 mM triethanolamine chloride, 50 mM sodium chloride, 1% BSA, pH 8.0 or another suitable buffer
Recertification 2 years
Storage 2-8°C
Synonyms NADP(+)-ICDH, NADP, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase
CAS Number 9028-48-2
E.C. Number
Reaction Isocitrate + NADP(+) <=> 2-oxoglutarate + CO(2) + NADPH