Isocitrate Dehydrogenase (IDH, NADP+)
Product Name | Isocitrate Dehydrogenase |
Source | Porcine Heart |
Catalogue Number | 341-02 |
Form | Liquid |
Purity | Partially Purified |
Activity | > 10 U/mL (Kinetic, spectrophotometric) |
Unit Definition | One unit will catalyze the oxidation of one micromole of isocitrate and the reduction of one micromole of NADP per minute at pH 7.4 and 37°C. |
Protein | > 10 mg/mL (Coomassie) |
Specific Activity | > 1 U/mg protein |
Formulation | Suspension in 3.3 M ammonium sulfate, 50 mM MES and 1 mM manganese sulfate, pH 6.4. |
Appearance | Brown suspension |
Handling | - Mix thoroughly before sampling - Dilute with 100 mM triethanolamine chloride, 50 mM sodium chloride, 1% BSA, pH 8.0 or another suitable buffer |
Recertification | 2 years |
Storage | 2-8°C |
Synonyms | NADP(+)-ICDH, NADP, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase |
CAS Number | 9028-48-2 |
E.C. Number | 1.1.1.42 |
Gene | IDH1, ICDH |
Reaction | Isocitrate + NADP(+) <=> 2-oxoglutarate + CO(2) + NADPH |
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