Isocitrate Dehydrogenase (IDH, NADP+)
| Product Name | Isocitrate Dehydrogenase |
| Source | Porcine Heart |
| Catalogue Number | 341-02 |
| Form | Liquid |
| Purity | Partially Purified |
| Activity | > 10 U/mL (Kinetic, spectrophotometric) |
| Unit Definition | One unit will catalyze the oxidation of one micromole of isocitrate and the reduction of one micromole of NADP per minute at pH 7.4 and 37°C. |
| Protein | > 10 mg/mL (Coomassie) |
| Specific Activity | > 1 U/mg protein |
| Formulation | Suspension in 3.3 M ammonium sulfate, 50 mM MES and 1 mM manganese sulfate, pH 6.4. |
| Appearance | Brown suspension |
| Handling | - Mix thoroughly before sampling - Dilute with 100 mM triethanolamine chloride, 50 mM sodium chloride, 1% BSA, pH 8.0 or another suitable buffer |
| Recertification | 2 years |
| Storage | 2-8°C |
| Synonyms | NADP(+)-ICDH, NADP, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase |
| CAS Number | 9028-48-2 |
| E.C. Number | 1.1.1.42 |
| Gene | IDH1, ICDH |
| Reaction | Isocitrate + NADP(+) <=> 2-oxoglutarate + CO(2) + NADPH |
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