Glutamate Dehydrogenase (GLDH)

Product Name	Glutamate Dehydrogenase
Source	Bovine Liver
Catalogue Number	260-10
Purity	Purified
Form	Lyophilized
Activity	> 15 U/mg
Unit Definition	One unit catalyzes the oxidation of one micromole of NADH and the reductive amination of one micromole of alpha-ketoglutarate per minute at 37°C and pH 7.95 in the presence of ADP.
Protein	> 0.25 mg protein/mg (Coomassie)
Specific Activity	> 20 U/mg protein
Contaminants	AST/SGOT: < 5% ALT/GPT: < 5% Ammonia: < 0.1 micromole/mg
Formulation	Lyophilized from sodium citrate and mannitol buffer.
Appearance	Tan powder
Recertification	3 years
Storage	-20°C
Application	Used for the determination of Urea in serum or plasma assays.
Function	Glutamate dehydrogenase is used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.
Synonyms	NAD(P)+, GDH1, L-GDH, GDH, Glutamate dehydrogenase 1, mitochondrial, L-Glutamate:NAD(P)+ oxidoreductase
Molecular Weight	~350,000
CAS Number	9029-12-3
E.C. Number	1.4.1.3
Gene	GLUD1, BOS_24262, GDH
Gene ID	281785
Accession No	P00366