Alpha-Amylase
| Product Name | Alpha-Amylase |
| Source | Human Saliva |
| Catalogue Number | 120-10 |
| Purity | > 90% (SDS-PAGE) |
| Purity Note | Typically > 98% (SDS-PAGE, two bands at approx. 60kDa) |
| Form | Lyophilized |
| Activity | > 100 U/mg (Siemens Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C. |
| Protein | > 0.2 mg protein/mg (Coomassie) |
| Specific Activity | > 400 U/mg protein |
| Contaminants | Lipase: < 0.05% Protease: < 0.1% Ammonia: < 0.1 micromole/mg |
| pH | 6.0 - 7.5 (10 mg/mL H2O) |
| Formulation | Lyophilized from Tris Chloride, Mannitol pH 7.2 |
| Appearance | White to Off-white powder |
| Solubility | Clear, colorless (10 mg/mL saline) |
| Reconstitution | Saline at ≥ 100 units/mL. Further dilutions should be in the lyophilization buffer (75 mM sodium chloride, 10 mM tris chloride, 1 mM calcium chloride, pH 7.2) or a similar solution. Calcium is important for the best activity and stability. |
| Related Products | 120-17 - α-Amylase from human saliva - Liquid 400-20 - Lipase from human pancreas |
| Storage | -20°C |
| Recertification | 3 years |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods. |
| Synonyms | 1,4-alpha-D-Glucan-glucanohydrolase, ptyalin |
| Molecular Weight | ~60,000 |
| CAS Number | 9000-90-2 |
| E.C. Number | 3.2.1.1 |
| Gene | AMY1A |
| Gene ID | 276 |
| Accession No | P04745 |
| References | Journal of Biosciences, 2013, Volume 38, Number 2, Page 259 |
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