Neutrophil Elastase (ELA-2)
| Product Name | Elastase |
| Source | Human Neutrophils |
| Catalogue Number | 342-45 |
| Purity | > 96% (SDS-PAGE) |
| Form | Lyophilized |
| Protein | Reported in mg/vial or mg protein/mg solid (Coomassie) |
| Activity | Reported |
| Unit Definition | One unit will hydrolyze one micromole of methoxy succinyl-alanine-alanine-proline-valine-p-nitroanilide per minute at 37°C and pH 7.5. |
| Specific Activity | > 10 U/mg protein |
| Formulation | Lyophilized from sodium acetate, pH 5.5. |
| Reconstitution | > 1 mg protein/mL in a buffer such as tris buffered saline, pH 7.5. Further dilutions should include a carrier such as 1% BSA. |
| Solubility | Clear, colorless to slightly hazy (saline) |
| Related Products | 550-21 - Troponin T antigen from human heart 431-11 - Myoglobin antigen from human heart 426-10 - Myeloperoxidase antigen from human leukocytes |
| Storage | 2-8°C |
| Recertification | 3 years |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HCV NAT, HIV-1 NAT and syphilis by FDA approved methods. |
| Synonyms | ELA-2, Elastase, HNE, NEG |
| CAS Number | 9004-06-2 |
| E.C. Number | 3.4.21.37 |
| Gene | ELANE, ELA2, GE, HLE, HNE, NE, PMN-E, SCN1 |
| Gene ID | 1991 |
| Accession No | P08246 |
Lee Biosolutions is the leading producer of human neutrophil Elastase (NEG) for clinical research, life science and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Human Neutrophils, also known as neutrophilic polymorphonuclear leukocytes, are the major class of white blood cell in human peripheral blood. They, have a multilobed nucleus and neutrophilic granules, and are phagocytes and have an important role in engulfing and killing extracellular pathogens.
The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains. Current studies show that both Cathepsin G and Neutrophil Elastase are key enzymes for tissue injury caused by activated neutrophils, such as occurs in Acute Lung injury.
Lee Biosolutions, Inc
