Alkaline Phosphatase (ALP)

Catalogue Number: 486-10
Product Name Alkaline Phosphatase
Source Human Liver
Catalogue Number 486-10
Purity Purified
Form Liquid
Activity > 25 U/mL (Dimension® Clinical Chemistry System)
Unit Definition One unit will catalyze the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 37°C and pH 10.35 in the presence of 2-amino-2-methyl-1-propanol.
Protein > 5 mg protein/mL (Coomassie)
Specific Activity Reported in U/mg protein
Formulation Suspension in 3.1 M ammonium sulfate with tris chloride, magnesium and zinc, pH 7.4.
Appearance Tan to off-white suspension
Recovery Centrifuge briefly to assure complete recovery. Mix thoroughly before sampling.
Storage 2-8°C
Recertification 2 years
CAS Number 9001-78-9
E.C. Number
Synonyms ALP, HL-ALP
Gene ID 249

Lee Biosolutions is the leading supplier of Human Liver Alkaline Phosphatase (ALP) for life science, laboratory, research and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available, email for more details.

Alkaline Phosphatase (ALP) is a hydrolase enzyme responsible for removing phosphate groups in the 5- and 3- positions from many types of molecules, including nucleotides, proteins, and alkaloids.

In humans, alkaline phosphatase is present in all tissues throughout the entire body, but is particularly concentrated in liver, bile duct, kidney, bone, and the placenta. The optimal pH for the enzyme activity is pH=10 in standard conditions.

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486-10-5 Inquire 5,000 units