Glycerol-3-Phosphate Dehydrogenase (GPDH)

Product Name Glycerol-3-Phosphate Dehydrogenase
Source Rabbit Muscle
Catalogue Number 276-50
Purity Purified
Form Lyophilized
Activity > 15 U/mg solid
Unit Definition One unit will catalyze the reduction of one micromole of dihydroxyacetone phosphate to alpha-glycerophosphate per minute at pH 7.4 and 25°C.
Protein > 0.3 mg protein/mg (Coomassie)
Specific Activity > 100 U/mg protein
Contaminants LDH: < 0.3%
PK: < 0.3%
TPI: < 10%
Aldolase: < 0.05%
Glycerol Kinase: < 0.01
Formulation Lyophilized powder from 0.05 M citrate, pH 6.5, with trehalose and PEG 20,000.
Appearance White to off-white powder
Reconstitution 5 - 10 mg/mL in saline
Storage -20°C
Recertification 3 years
Heat Inactivation GDH is rapidly inactivated at temperatures > 65°C
Application This enzyme is useful for enzymatic determination of glycerol when coupled with glycerol kinase in clinical analysis.
Synonyms GPDH, G3PDH, GPD, Glycerophosphate Dehydrogenase
Molecular Weight 75,200
CAS Number 9075-65-4
E.C. Number
Accession No P08507
Gene GPD1