Glycerol-3-Phosphate Dehydrogenase (GPDH)

Product Name	Glycerol-3-Phosphate Dehydrogenase
Source	Rabbit Muscle
Catalogue Number	276-50
Purity	Purified
Form	Lyophilized
Activity	> 15 U/mg solid
Unit Definition	One unit will catalyze the reduction of one micromole of dihydroxyacetone phosphate to alpha-glycerophosphate per minute at pH 7.4 and 25°C.
Protein	> 0.3 mg protein/mg (Coomassie)
Specific Activity	> 100 U/mg protein
Contaminants	LDH: < 0.3% PK: < 0.3% TPI: < 10% Aldolase: < 0.05% Glycerol Kinase: < 0.01
Formulation	Lyophilized powder from 0.05 M citrate, pH 6.5, with trehalose and PEG 20,000.
Appearance	White to off-white powder
Reconstitution	5 - 10 mg/mL in saline
Storage	-20°C
Recertification	3 years
Heat Inactivation	GDH is rapidly inactivated at temperatures > 65°C
Application	This enzyme is useful for enzymatic determination of glycerol when coupled with glycerol kinase in clinical analysis.
Synonyms	GPDH, G3PDH, GPD, Glycerophosphate Dehydrogenase
Molecular Weight	75,200
CAS Number	9075-65-4
E.C. Number	1.1.1.8
Accession No	P08507
Gene	GPD1