Transferrin, Holo
| Product Name | Holo Transferrin |
| Source | Human Plasma/Serum |
| Catalogue Number | 535-11 |
| Purity | > 98% (cellulose acetate electrophoresis) |
| Form | Lyophilized |
| Iron Content | 1,200 - 1,700 ppm (ICP) |
| Heat Treatment | Product held at > 58°C for > 10 hours. |
| Moisture | < 5% (Karl Fischer) |
| pH | 6.5 - 8.0 (3% solution) |
| Endotoxin | < 1 EU/mg |
| Bioburden | < 10 CFU/mL |
| Formulation | Lyophilized from ammonium bicarbonate, may contain traces of buffer salts. |
| Related Products | 535-10 - Sidero Transferrin from human plasma 535-12 - Apo Transferrin from human plasma 535-70R - Recombinant Human Transferrin |
| Storage | 2-8°C |
| Recertification | 5 years |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HCV NAT, HIV-1 NAT and syphilis by FDA approved methods. |
| Synonyms | Transferrin iron-saturated, Siderophilin, TRF, Holo TnF |
| Molecular Weight | 77,000 |
| CAS Number | 11096-37-0 |
| E.C. Number | 234-318-8 |
Lee Biosolutions is the leading supplier of Human Holo Transferrin from human serum, used for cell culture, medical research and manufacturing of clinical diagnostic assays for iron deficiencies and disorders of the immune system.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Purification procedures include selective size exclusion, ion exchange chromatography and gel fitration for transferrin products.
Ultra Pure Human Holo Transferrin >99% pure available upon request.
Human Transferrin is a plasma protein for iron ion delivery. Human Transferrin is a glycoprotein with homologous N-terminal and C-terminal iron binding domains. Transferrin (TF) is related to other iron binding proteins including lactoferrin. When human transferrin loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and is consequently transported into the cell in a vesicle. The cell will acidify the vesicle, causing human transferrin(TF) to release its iron ions. Each human transferrin molecule has the ability to carry two iron ions in the ferric form (Fe3+).
Human Transferrin levels may be checked in iron deficiency, hemochromatosis and other iron overload disorders. Transferrin is also associated with the innate immune system. Human Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron, where few bacteria are able to survive.
Lee Biosolutions, Inc
