Kappa Light Chain (Bound + Free), anti-Human
Catalogue Number: GKBF-80A
Description
Product Name | anti-Human Bound + Free Kappa Chain |
Source | Polyclonal Antibody |
Purity | Affinity Purified |
Catalogue Number | GKBF-80A |
Form | Liquid |
Protein | 1 - 2 mg/mL (A280, E0.1%= 1.4) |
Host | Goat |
Isotype | IgG |
Immunogen | Highly purified kappa chains from human IgG |
Specificity | The affinity purified anti-kappa reacts with IgG kappa chains.It does not react with IgG lambda chains. Suitable for IP, ELISA and blotting applications. |
Antibody Type | Secondary Antibody |
Formulation | Solution in phosphate buffered saline pH 7.2 with 0.09% sodium azide. |
Related Products | 410-12 - Kappa Free Light Chain antigen GKGF-80A - anti-Human Bound + Free Kappa Light Chain Polyclonal Antibody GLBF-80A - anti-Human Bound + Free Lambda Light Chain Polyclonal Antibody |
Storage | 2-8°C |
Recertification | 2 years |
Synonyms | anti-KLC |
Applications
Overview
Polyclonal anti-human Kappa procedure: Goats were immunized with highly purified human kappa chains from normal IgG. Following immunization in Freund's adjuvant the goats were bled, serum collected and the antiserum absorbed with a lambda containing immunoabsorbant. The anti kappa was isolated from an IgG kappa affinity column
The affinity purified anti kappa reacts with both bound and free kappa chains. It does not react with bound or free lambda chains by IEP and double diffusion analysis.
Western Blots 1:5,000-1-10,000 ELISA coating 1:100-1:500
Inquire on our custom polyclonal antibody production. We can host from any species and cross link to your requirements. Custom Polyclonal antibody concentrations available.
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