Aspartate Aminotransferase (AST, GOT)
Catalogue Number: 300-20
Description
| Product Name | Aspartate Aminotransferase |
| Source | Porcine Heart |
| Catalogue Number | 300-20 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 100 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8 |
| Protein | > 0.7 mg protein/mg solid (A280) Report mg protein/mg solid (Coomassie) |
| Specific Activity | > 110 U/mg protein (A280) Report U/mg protein (Coomassie) |
| Contaminants | CPK: < 0.1% GPT/ALT: < 0.1% LDH: < 0.1% GLDH: < 0.1% MDH: < 0.01% Protease: < 0.1% Ammonia: < 0.005 micromole/mg |
| Formulation | Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0. |
| Appearance | Yellow to yellow-green powder |
| Reconstitution | > 1 mg/mL in tris buffered saline, 1% BSA, pH 8.0. |
| Isoform | Cytosolic |
| Related Products | 480-20 - Alkaline Phosphatase from calf intestine 310-30 - Alanine Aminotransferase from porcine heart 350-50 - Lactate Dehydrogenase from chicken heart |
| Storage | -20°C |
| Recertification | 3 years |
| Application | Control, Calibrator, Research, Manufacturing |
| Synonyms | Aspartate Transaminase, glutamate oxaloacetate transaminase, AST, GOT, sGOT, AspAT, ASAT, AAT, serum glutamic oxaloacetic transaminase |
| Molecular Weight | ~92,000 (Feliss and martinez-Carrion 1970) |
| CAS Number | 9000-97-9 |
| E.C. Number | 2.6.1.1 |
| Gene | GOT1 |
| Accession No | P00503 |
Applications
Overview
Lee Biosolutions produces Porcine Heart Aspartate Transferase (AST), also known as Glutamate Oxaloacetate Transaminase (GOT) for research and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details. Single batch sizes from 100,000 kU.
There are two SGOT/AST isoenzymes are present in humans, they have high similarity. GOT1, the cytosolic isoenzyme derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.
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Lee Biosolutions, Inc
