Alpha-Amylase
| Product Name | Alpha-Amylase |
| Source | Human Saliva |
| Catalogue Number | 120-10 |
| Purity | > 90% (SDS-PAGE) |
| Purity Note | Typically > 98% (SDS-PAGE, two bands at approx. 60kDa) |
| Form | Lyophilized |
| Activity | > 100 U/mg (Siemens Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole 2-chloro-4-nitrophenyl-a-D-maltotrioside to yield 2-chloro-4-nitrophenol per minute at 37°C. |
| Protein | > 0.2 mg protein/mg (Coomassie) |
| Specific Activity | > 400 U/mg protein |
| Contaminants | Lipase: < 0.05% Protease: < 0.1% Ammonia: < 0.1 micromole/mg |
| pH | 6.0 - 7.5 (10 mg/mL H2O) |
| Formulation | Lyophilized from Tris Chloride, Mannitol pH 7.2 |
| Appearance | White to Off-white powder |
| Solubility | Clear, colorless (10 mg/mL saline) |
| Reconstitution | Saline at ≥ 100 units/mL. Further dilutions should be in the lyophilization buffer (75 mM sodium chloride, 10 mM tris chloride, 1 mM calcium chloride, pH 7.2) or a similar solution. Calcium is important for the best activity and stability. |
| Related Products | 120-17 - α-Amylase from human saliva - Liquid 400-20 - Lipase from human pancreas |
| Storage | -20°C |
| Recertification | 3 years |
| Infectious Disease Testing | Negative or non-reactive at the donor level for anti-HIV 1 and 2, anti-HCV, HBsAg, HIV-1 (NAT), HBV (NAT), HCV (NAT), and syphilis by FDA approved methods. |
| Synonyms | 1,4-alpha-D-Glucan-glucanohydrolase, ptyalin |
| Molecular Weight | ~60,000 |
| CAS Number | 9000-90-2 |
| E.C. Number | 3.2.1.1 |
| Gene | AMY1A |
| Gene ID | 276 |
| Accession No | P04745 |
| References | Journal of Biosciences, 2013, Volume 38, Number 2, Page 259 |
Lee Biosolutions produces and manufactures high purity human salivary Amylase for research and diagnostic manufacturing uses.
Custom preparations, technical support, bulk quantities and aliquoting available, email Info@leebio.com for more details.
Amylase is an enzyme that breaks starch down into sugar. Amylase is present in human saliva, where it begins the chemical process of digestion. Foods that contain much starch but little sugar, such as rice and potato, taste slightly sweet as they are chewed because amylase turns some of their starch into sugar in the mouth.
Alpha-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides such as starch and glycogen, yielding glucose and maltose. It is being used by researchers as a barometer of the body's response to physical or psychological stress. NIH study shows that Women with high levels of alpha amylase, indicating stress, are less likely to conceive.
Lee Biosolutions, Inc
